Does the compressor engage when you turn your dash air on with the engine running? If not, then you may be low on refrigerant or there could be an electrical problem preventing the compressor from engaging.
You mention hooking a gauge to the low pressure side. What kind of gauge are you using and are you sure you're connected to the right port?
The R134a ports do have one-way valves in them similar to a schrader valve, but I don't think they're removable. An R134a A/C manifold gauge coupler is a quick connect type that has a knob on it that when turned will depress the valve inside the port to the system.
I'm not sure I fully understand where you're connecting to, but if you can give a bit more detail I'll be glad to try to help out.
Also, being that your coach is a 2004, you may still be under Workhorse's factory 3yr./36k mile chassis warranty. If there's a leak in the A/C system that's part of the chassis it may be a covered repair.