No. Remember that inanimate objects are not effected by what warm blooded creatures call 'wind chill'. But...it's best that the wet bay doesn't have any big gaps so close or block any holes. Next, water has thermal mass, and it takes a powerful cold for a long while to freeze it. So when traveling in the cold, 1/3 tank or more is best. Next, you can buy gallon jugs of RV antifreeze that you can pour into empty gray and black tanks, drains and the toilet if you wish but I wouldn't unless I was going to park it for days in the cold without heat, next, those tanks are 'freeze resistant' and a thin layer of liquid at the bottom won't harm them, even if it freezes. Same with newer PEX plumbing.
Than, you can turn off the water pump and open the kitchen faucet, then open the low spot drain to get most of the drinking water out of the wet bay (which will be the coldest area) while you are traveling but only bother if it's going to be less the 20 F the entire trip. Close the valve after draining.
So that's it. These things are just preventative and if the temps are above 20 F for the trip, I wouldn't bother as your RV will withstand anything above that for DAYS.
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